2019-02-15
A Versatile Method to Quantify DNA-Protein Interactions on Negatively Supercoiled DNA
Publication
Publication
Biophys. J. , Volume 116 - Issue 3 p. 214a- 214a
Many genomic processes are regulated by torsional stress, resulting in a range of supercoiled DNA structures. In order to understand the effect of such structures on protein binding and activity, several single-molecule techniques are often employed. These include magnetic, micro-pipette and angular optical tweezers. However, two factors can limit the study of DNA-protein interactions on supercoiled DNA. First, it is challenging to combine DNA-torque control with fluorescence microscopy. Second, the DNA substrate is typically tethered to a surface, hindering rapid buffer exchange.
Additional Metadata | |
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Elsevier/ Cell Press | |
doi.org/10.1016/j.bpj.2018.11.1182 | |
Biophys. J. | |
Organisation | Biological Soft Matter-Former Group |
King, G., Burla, F., Peterman, E., & Wuite, G. (2019). A Versatile Method to Quantify DNA-Protein Interactions on Negatively Supercoiled DNA. In Biophys. J. (Vol. 116, pp. 214a–214a). doi:10.1016/j.bpj.2018.11.1182 |