2017-08-01
Ion mobility spectrometry reveals intermediate states in temperature-resolved DNA unfolding
Publication
Publication
Int. J. Mass Spectrom. , Volume 419 p. 52- 55
Electrospray ionization (ESI) mass spectrometry (MS) is used to study the non-covalent interactions of short 12-mer oligonucleotides under native conditions. A home-built, temperature-controlled ESI source is employed to elevate the spray temperature above the melting temperature (T-m) of the DNA duplexes, enabling the study of DNA interactions in the liquid phase rather than gas phase. Ion mobility spectrometry (IMS)-MS is used to investigate the 3-dimensional structure of the hybridized oligonucleotides and to track conformational changes in the oligonucleotide duplexes during temperature-induced melting in solution. Results show two additional drift times at T > 35 degrees C, indicating 2-fold (partial) unfolding dynamics for the DNA duplex with stable intermediates. (C) 2017 Elsevier B.V. All rights reserved.
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| , , , | |
| Elsevier B.V. | |
| The Netherlands Organisation for Scientific Research (NWO) | |
| doi.org/10.1016/j.ijms.2017.03.008 | |
| Int. J. Mass Spectrom. | |
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Hommersom, B., Siegel, T. P., & Heeren, R. (2017). Ion mobility spectrometry reveals intermediate states in temperature-resolved DNA unfolding. Int. J. Mass Spectrom., 419, 52–55. doi:10.1016/j.ijms.2017.03.008 |
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