Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions
Nature Commun. , Volume 14 - Issue 1 p. 4345: 1- 8
DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, with the reported implementation, only a single target can be imaged and the technique cannot be applied straight to live cell imaging. Here we report on leveraging this technique from a proof-of-principle implementation to a useful tool for the SPT community by introducing simultaneous live cell dual-color DNA-PAINT-SPT for quantifying protein dimerization and tracking proteins in living cell membranes, demonstrating its improved performance over single-dye SPT.
|NWO VIDI , NWO WISE|
|Organisation||Physics of Cellular Interactions|
Niederauer, C, Nguyen, C, Wang-Henderson, M, Stein, J, Strauss, S, Cumberworth, A, … Ganzinger, K.A. (2023). Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions. Nature Commun., 14(1), 4345: 1–4345: 8. doi:10.1038/s41467-023-40065-8