A desynchrony mechanism between events triggers a compensatory delay during Caenorhabditis elegans development

In multicellular organisms, development entails the progression of diverse cellular processes that need to be temporally coordinated. During Caenorhabditis elegans postembryonic development, the events of molting and cell divisions progress in parallel during four larval stages and are modulated by external cues such as temperature and nutrient availability. While seam cell divisions occur predominantly before ecdysis of the cuticle, the order of the events can change, suggesting that they are controlled by independent mechanisms. Here, we have analyzed the impact of reduced insulin signaling on molting and in stage-specific cell divisions. We find that reduced insulin signaling in the daf-2(e1370) allele delays both events but has a larger impact on the timing of cell divisions, thus increasing the desynchrony between the events. The relative delay in seam cell divisions leads to a delay in the initiation of the subsequent stage of the molting program, providing a mechanism for resynchronization of developmental processes at the beginning of the larval stages.

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