Previous experiments have shown that the anatomy and chemical composition of leaves of inherently fast- and slow-growing grass species, grown at non-limiting nitrogen supply, differ systematically. The present experiment was carried out to investigate whether these differences persist when the plants are grown at an intermediate or a very low nitrogen supply. To this end, the inherently fast-growing Poa annua L. and Poa trivialis L., and the inherently slow-growing Poa compressa L. and Poa pratensis (L.) Schreb. were grown hydroponically at three levels of nitrate supply: at optimum (RGRmax) and at relative addition rates of 100 and 50 mmol N (mol N-1 d-1 (RAR100 and RAR50), respectively.

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Journal Plant, Cell Environ.
van Arendonk, J. J. C. M, Niemann, G. J, Boon, J. J, & Lambers, H. (1997). Effects of nitrogen supply on the anatomy and chemical composition of leaves of four grass species belonging to the genus Poa, as determined by image-processing analysis and pyrolysis-mass spectrometry. Plant, Cell Environ., 20, 881–897.