We present a new procedure that allows for the controlled patterning of functional proteins on the sidewalls of three-dimensional microfabricated chambers. We build microchambers with walls containing gold areas of a defined submicrometer size, to which proteins can be bound specifically, via thiol chemistry. We tested our system by observing the gliding of microtubules along motor-coated microchamber sidewalls and find that introducing an intermediate multilayer of proteins greatly enhances motor protein activity. This combination of spatial confinement and three-dimensional patterning of protein activity opens up new possibilities for the in vitro study of cellular processes, as well as for the development of guided transport mechanisms in nanodevices

Nano Lett.

Romet-Lemonne, G., van Duijn, M. M., & Dogterom, M. (2005). Three-dimensional control of protein patterning in microfabricated devices. Nano Lett., 5, 2350–2354. doi:10.1021/nl0507111