TOF-SIMS is popular in biological sciences due to its ability to investigate the spatial distribution of molecules in tissue sections and other biological samples with a very high spatial resolution. Here we present an example of a strategy that determines the molecular distribution of cholesterol on the surface of a tissue section. In this chapter several sample preparation considerations for TOF-SIMS analysis are presented. Additionally, the influence of different sample coating methods and technical obstacles, which may appeared during analysis, will be discussed.

Additional Metadata
Publisher NOVA Publisher
Editor S. König
Citation
Bodzon-Kulakowska, A, Kiss, A, Chughtai, K, & Heeren, R.M.A. (2012). Distribution of cholesterol in the brain tissue as an example of TOF-SIMS analysis. In S König (Ed.), 8th Münster Conference on Single Cell and Molecule Analysis Nov. 16-17, 2011 : Proceedings (pp. 1–10). NOVA Publisher.