Septins promote F-actin ring formation by cross-linking actin filaments into curved bundles
Nature Cell Biol. , Volume 16 p. 322- 334
Animal cell cytokinesis requires a contractile ring of crosslinked actin filaments and myosin motors. How contractile rings form and are stabilized in dividing cells remains unclear. We address this problem by focusing on septins, highly conserved proteins in eukaryotes whose precise contribution to cytokinesis remains elusive. We use the cleavage of the Drosophila melanogaster embryo as a model system, where contractile actin rings drive constriction of invaginating membranes to produce an epithelium in a manner akin to cell division. In vivo functional studies show that septins are required for generating curved and tightly packed actin filament networks. In vitro reconstitution assays show that septins alone bundle actin filaments into rings, accounting for the defects in actin ring formation in septin mutants. The bundling and bending activities are conserved for human septins, and highlight unique functions of septins in the organization of contractile actomyosin rings.
|Nature Cell Biol.|
|Organisation||Biological Soft Matter-Former Group|
Mavrakis, M, Azou-Gros, Y, Tsai, F-C, Alvarado, J, Bertin, A, Iv, F, … Lecuit, T. (2014). Septins promote F-actin ring formation by cross-linking actin filaments into curved bundles. Nature Cell Biol., 16, 322–334. doi:10.1038/ncb2921