The kinetics of a key noncovalent, hydrogen bonding interaction was studied in situ using coherent anti-stokes Raman scattering (CARS) microspectroscopy in a microfluidic device. The association of model compounds, pyridine and hexafluoroisopropanol, was quantitatively monitored with submicrometer resolution. Lower limits for the very high formation and dissociation rate constants of the model 1:1 pyridine–hexafluoroisopropanol hydrogen bonded complex in dichloromethane-d2 were determined to be k1 > 105 M–1s–1 and k–1 > 333.3 s–1, respectively.

Anal. Chem.

Oshovsky, G. V., Rago, G., Day, J. P. R., Soudijn, M. L., Rock, W., Parekh, S. H., … Bonn, M. (2013). Coherent anti-stokes Raman Scattering microspectroscopic kinetic study of fast hydrogen bond formation in microfluidic devices. Anal. Chem., 85(19), 8923–8927. doi:10.1021/ac402233n