2017-07-20
Nano-antenna enhanced two-focus fluorescence correlation spectroscopy
Publication
Publication
Sci. Rep. , Volume 7 - Issue Article number: 5985 p. 1- 9
We propose two-focus fluorescence correlation spectroscopy (2fFCS) on basis of plasmonic nanoantennas that provide distinct hot spots that are individually addressable through polarization, yet lie within a single diffraction limited microscope focus. The importance of two-focus FCS is that a calibrated distance between foci provides an intrinsic calibration to derive diffusion constants from measured correlation times. Through electromagnetic modelling we analyze a geometry of perpendicular nanorods, and their inverse, i.e., nanoslits. While we find that nanorods are not suited for nano-antenna enhanced 2fFCS due to substantial background signal, a nanoslit geometry is expected to provide a di tinct cross-correlation between orthogonally polarized detection channels. Furthermore, by utilizing a periodic array of nanoslits instead of a single pair, the amplitude of the cross-correlation can be enhanced. To demonstrate this technique, we present a proof of principle experiment on the basis of a periodic array of nanoslits, applied to lipid diffusion in a supported lipid bilayer.
Additional Metadata | |
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European Research Council (ERC) , NWO | |
NPG | |
doi.org/10.1038/s41598-017-06325-6 | |
Sci. Rep. | |
NanoNextNL | |
Organisation | Biological Soft Matter-Former Group |
Langguth, L., Szuba, A., Mann, S., Garnett, E., Koenderink, G., & Koenderink, F. (2017). Nano-antenna enhanced two-focus fluorescence correlation spectroscopy. Sci. Rep., 7(Article number: 5985), 1–9. doi:10.1038/s41598-017-06325-6 |