We formulate, implement and test a robust method of determining sphere size distributions from finite thickness planar sections. The method uses a forward approach in which populations of proposed distributions are tested against the input data and refined using a genetic algorithm. This method is then applied to a real-world data set concerning endo- and exocytotic vesicles in the apical region of tip growing pollen tubes of Arabidopsis thaliana.

J. Microsc.
Theory of Biomolecular Matter

de Nooijer, S, Ketelaar, T, & Mulder, B.M. (2008). Sphere size distributions from finite thickness sections: a forward approach employing a genetic algorithm. J. Microsc., 231, 257–264. doi:10.1111/j.1365-2818.2008.02032.x